Sergey Tumanov

Sergey Tumanov

PhD Student

School of Biological Sciences, The University of Auckland

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RESEARCH INTERESTS

  • Metabolomics and lipidomics
  • Food microbiology
  • Bioorganic and bioanalytical chemistry
  • Mass spectrometry
  • Bioconjugation and chemistry of biocionjugates
  • Bioinformatics and statistics

 

BACKGROUND

  • 2011 - PhD student at Metabolomics Lab, School of Biological Sciences, The University of Auckland
  • 2010-2011 – Research Assistant at Auckland UniServices
  • 2010 – MSc in Chemical Sciences, Belarusian State University, Minsk, Belarus
  • 2009 – Specialist Diploma in Analytical Chemistry and Methods of Analysis

 

CURRENT PROJECT

1. Method development for absolute non-targeted metabolite quantification through introduction of mass (isotopic) tags followed by GC-MS analysis

Methods for metabolite profiling are the most common analytical approach in metabolomics. However, most methods require internal standardization to correct technical variability imposed mainly by variations in matrix composition. Moreover, metabolomics pursues unbiased and non-targeted metabolite analysis aiming the absolute quantitation. Chemical derivatization of metabolites is a very useful tool for introducing the isotopic label alongside with their volatilization. Labelled metabolite derivatives then play the role of internal standards for absolute quantification. Developed method was applied for both metabolite and fatty acid absolute quantification in variety of biological samples (Fig.1). Method was validated using grape juice and wine as a part of my research in New Zealand Sauvignon Blanc II Program and Juice Index.

Sample preparation workflow for absolute quantitative approach

Figure 1. Sample preparation workflow for absolute quantitative approach

 

2. Modified fluorescent dyes for glycerophospholipid absolute quantification using high resolution mass spectrometry

Glycerophospholipid molecules are building blocks of the cellular membrane and play a key role in the biochemistry of cells. ESI-MS has become an appropriate analytical platform for the analysis of multicomponent lipid fractions from biological samples due to its higher sensitivity and capacity for high throughput analysis. However, the absolute quantification of certain subclasses of glycerophospholipids is linear within limited range of concentrations and usually requires the introduction of synthetic lipid standards.

A new potentially improved approach for absolute non-targeted glycerophospholipid quantification is based on the conjugation of lipid molecules with a modified fluorescent dye followed by liquid chromatography separation and mass spectrometry analysis. This approach has a potential to expand the linear concentration range for quantification, avoiding introduction of inorganic ions and shifting the masses of molecular ions of lipid-dye conjugates to the range of 1000-1600 Da (Fig.2).

A new potentially improved approach for absolute non-targeted glycerophospholipid quantification based on the conjugation of lipid molecules with a modified fluorescent dye

Figure 2. A new potentially improved approach for absolute non-targeted glycerophospholipid quantification based on the conjugation of lipid molecules with a modified fluorescent dye

 

SELECTED PUBLICATIONS

  • Han T-L, Tumanov S, Cannon RD, Villas-Boas SG (2013) Metabolic Response of Candida albicans to Phenylethyl Alcohol under Hyphae-Inducing Conditions. PLoS ONE 8(8): e71364. doi:10.1371/journal.pone.0071364 >>>

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